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당뇨쥐 상악골에서 즉시 임플란트 후 골유착에 대한 면역조직화학적 연구
Immunohistochemical Study of Bone Response to the Immediate Implantation in the Rat Maxilla

대한통합치과학회지 2017년 6권 2호 p.39 ~ 45

조창준, 박수현, 허현아, 신현정, 최재호, 표성운,

소속 상세정보

조창준 ( Cho Chang-Joon ) - 가톨릭대학교 임상치과학대학원 구강악안면외과
박수현 ( Park Su-Hyun ) - 가톨릭대학교 의과대학 치과학교실 구강악안면외과
허현아 ( Heo Hyun-A ) - 가톨릭대학교 의과대학 치과학교실 구강악안면외과
신현정 ( Shin Hyun-Jung ) - 가톨릭대학교 의과대학 부천성모병원 치과
최재호 ( Choi Jae-Ho ) - 가톨릭대학교 의과대학 부천성모병원 치과
표성운 ( Pyo Sung-Woon ) - 가톨릭대학교 의과대학 치과학교실 구강악안면외과

KMID : 1146620170060020039

Abstract

Purpose: Dental implants are used successfully for restoration of dental function. However, the impact of diabetes on the healing of bone associated with immediately placed dental implants is not completely understood. And little is known about the expression of biological molecules involved in bone healing around implants. The purpose of this study was to investigate the bone response around immediately placed titanium implants in the maxilla of ovariectomized rats by immunohistochemical method.

Materials and methods: 36 rats were divided into the control, insulin-treated and diabetic groups. Rats received streptozotocin (60 mg/kg) to induce diabetes; animals in the insulin-treated group also received 3 units of subcutaneous slow-release insulin. Two titanium implants (1.2×3 mm) were placed in the extraction socket of maxillary first molars of animals and specimens were harvested at 3 days, 1, 2 and 4 weeks. The expression levels of bone morphogenetic protein (BMP)-4, transforming growth factor (TGF)-β and osteonectin (ON) were measured.

Result: The BMP-4 expression increased immediately all groups at day 3, week 1 and week 2. However, staining intensity of diabetic group was weaker than the control and the insulin-treated group but not significant. At week 4, in all groups, the mount of BMP-4 expression decreased. The TGF-β expression instantly showed at day 3 in control group only. At week 1 and 2, TGF-β staining gradually increased in all group. But in the all group staining intensity decreased at 4 week. And we found the expression of ON in the control and the insulin-treated group were higher than that of the diabetic group at 1 weeks and 4 weeks, indicated that bone formation be active in those groups.

Conclusion: In this study, insulin treatment increased expression of biologic molecules in the bone around the immediate placed titanium implants in the maxilla of diabetic rat. It is expected that insulin might reverse the reduced predictability of success of immediate implantation with the uncontrolled diabetes patients.